Difference between revisions of "Part:BBa K5115001"

Revision as of 15:30, 24 September 2024

hoxF, without stop codon

It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system BBa_K5115011(ribozyme+RBS+hoxF+stem-loop). And then it will eventually make up parts BBa_K5115066(mineral, U module)and BBa_K5115067(mineral, F module), which are used for further experiment.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 577
Illegal BglII site found at 1395
Illegal BglII site found at 1688
Illegal XhoI site found at 22
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 456
Illegal NgoMIV site found at 994
Illegal NgoMIV site found at 1204
Illegal NgoMIV site found at 1516
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 625
Illegal BsaI.rc site found at 1129

@@ Line 6: / Line 6: @@
 __TOC__
 ===Introduction===
-The hoxU is a subunit of the hox and hyp operon [https://parts.igem.org/Part:BBa_K5115020 BBa_K5115020].
+The hoxF is a subunit of the hox and hyp operon [https://parts.igem.org/Part:BBa_K5115020 BBa_K5115020].
 ===Usage and Biology===
-It's used to buid up the hydrogenase which ruduces the nickel ion. In our experiment, it was construct into our ribozyme-assisted polycistronic co-expression system [https://parts.igem.org/Part:BBa_K5115012 BBa_K5115013]
+It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system [https://parts.igem.org/Part:BBa_K5115011 BBa_K5115011(ribozyme+RBS+hoxF+stem-loop)]. And then it will eventually make up parts [https://parts.igem.org/Part:BBa_K5115066 BBa_K5115066(mineral, U module)]and [https://parts.igem.org/Part:BBa_K5115067 BBa_K5115067(mineral, F module)], which are used for further experiment.
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