Difference between revisions of "Part:BBa K5115001"
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<partinfo>BBa_K5115001 short</partinfo> | <partinfo>BBa_K5115001 short</partinfo> | ||
− | <html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM | + | <html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM 2024"></html> |
__TOC__ | __TOC__ | ||
===Introduction=== | ===Introduction=== | ||
− | The | + | The hoxU is a subunit of the hox and hyp operon [https://parts.igem.org/Part:BBa_K5115020 BBa_K5115020]. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | It's used to buid up the hydrogenase which ruduces the nickel ion. In our experiment, it was put into the ribozyme system [https://parts.igem.org/Part:BBa_K5115011 BBa_K5115011] in case that it can be expressed smoothly in a polucostronic sequences. | |
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Revision as of 13:04, 24 September 2024
hoxF, without stop codon
Introduction
The hoxU is a subunit of the hox and hyp operon BBa_K5115020.
Usage and Biology
It's used to buid up the hydrogenase which ruduces the nickel ion. In our experiment, it was put into the ribozyme system BBa_K5115011 in case that it can be expressed smoothly in a polucostronic sequences.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 577
Illegal BglII site found at 1395
Illegal BglII site found at 1688
Illegal XhoI site found at 22 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 456
Illegal NgoMIV site found at 994
Illegal NgoMIV site found at 1204
Illegal NgoMIV site found at 1516 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 625
Illegal BsaI.rc site found at 1129