Difference between revisions of "Part:BBa K518011"

 
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<partinfo>BBa_K518011 parameters</partinfo>
 
<partinfo>BBa_K518011 parameters</partinfo>
 
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===General information of RecN provided by CUHK-HongKong-SBS 2024===
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Proteins belonging to the structural maintenance of chromosomes (SMC) family are conserved in both prokaryotic and eukaryotic cells, playing significant roles in the dynamics of chromosomes, such as chromosome cohesion, condensation, and DNA repair processes. In the case of Escherichia coli, at least three SMC-like proteins have been identified: MukB, SbcC, and RecN. The RecN gene in E.coli possesses three SOS boxes in the promoter region, and the RecN protein produced is specifically targeted for degradation by the ClpXP protease, which recognizes short signals present at its C terminus, leading to a very short half life of about 10 minutes (1). Thus, the regulation of RecN expression is governed by the LexA repressor and is confined to cells that have undergone DNA damage. The RecN protein is one of the most highly synthesized proteins activated during the SOS response to DNA damage, emphasizing its significant role in the DNA damage response pathway (2).
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===References===
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1. Graumann, P. L., & Knust, T. (2009). Dynamics of the bacterial SMC complex and SMC-like proteins involved in DNA repair. Chromosome Research, 17(2), 265–275. https://doi.org/10.1007/s10577-008-9014-x
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2. Reyes, E. D., Patidar, P. L., Uranga, L. A., Bortoletto, A. S., & Lusetti, S. L. (2010). RecN Is a Cohesin-like Protein That Stimulates Intermolecular DNA Interactions in Vitro. Journal of Biological Chemistry , 285(22), 16521–16529. https://doi.org/10.1074/jbc.m110.119164

Latest revision as of 09:03, 24 September 2024


recN promoter

Microbes including Escherichia coli are known to respond to various DNA-injuring stress (ionizing radiation, ultraviolet radiation, peroxides etc...), altering their gene expressions. This response, known as "SOS response", are induced by regulatory protein called RecA, which is activated when binds to single-strand DNA. DNA-RecA complex promotes the self-degradation of LexA, a common repressor for SOS genes. RecN is involved in repair of double strand breaks in the chromosome. The promoter of recN, or recNp, is acutely induced by various stress factors.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


General information of RecN provided by CUHK-HongKong-SBS 2024

Proteins belonging to the structural maintenance of chromosomes (SMC) family are conserved in both prokaryotic and eukaryotic cells, playing significant roles in the dynamics of chromosomes, such as chromosome cohesion, condensation, and DNA repair processes. In the case of Escherichia coli, at least three SMC-like proteins have been identified: MukB, SbcC, and RecN. The RecN gene in E.coli possesses three SOS boxes in the promoter region, and the RecN protein produced is specifically targeted for degradation by the ClpXP protease, which recognizes short signals present at its C terminus, leading to a very short half life of about 10 minutes (1). Thus, the regulation of RecN expression is governed by the LexA repressor and is confined to cells that have undergone DNA damage. The RecN protein is one of the most highly synthesized proteins activated during the SOS response to DNA damage, emphasizing its significant role in the DNA damage response pathway (2).

References

1. Graumann, P. L., & Knust, T. (2009). Dynamics of the bacterial SMC complex and SMC-like proteins involved in DNA repair. Chromosome Research, 17(2), 265–275. https://doi.org/10.1007/s10577-008-9014-x

2. Reyes, E. D., Patidar, P. L., Uranga, L. A., Bortoletto, A. S., & Lusetti, S. L. (2010). RecN Is a Cohesin-like Protein That Stimulates Intermolecular DNA Interactions in Vitro. Journal of Biological Chemistry , 285(22), 16521–16529. https://doi.org/10.1074/jbc.m110.119164