Difference between revisions of "Part:BBa K5317016:Design"
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Latest revision as of 16:59, 23 September 2024
ATF2
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 21
Illegal EcoRI site found at 283
Illegal XbaI site found at 324
Illegal PstI site found at 731
Illegal PstI site found at 1180 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 21
Illegal EcoRI site found at 283
Illegal PstI site found at 731
Illegal PstI site found at 1180 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 21
Illegal EcoRI site found at 283 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 21
Illegal EcoRI site found at 283
Illegal XbaI site found at 324
Illegal PstI site found at 731
Illegal PstI site found at 1180 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 21
Illegal EcoRI site found at 283
Illegal XbaI site found at 324
Illegal PstI site found at 731
Illegal PstI site found at 1180 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1031
Design Notes
This sequence was codon optimised for human cell lines.
Source
We obtained this sequence from S. aureus.