Difference between revisions of "Part:BBa K5317021:Design"
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===Source===
 
===Source===
  
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The fluorescent marker mRuby was obtained from Eurofins and ATF2 was acquired from S. aureus.  
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The fluorescent marker mRuby was obtained from Eurofins and ATF2 was acquired from S. aureus.
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===References===
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Kirsch, K., Zeke, A., Tőke, O., Sok, P., Sethi, A., Sebő, A., Kumar, G. S., Egri, P., Póti, Á. L., Gooley, P., Peti, W., Bento, I., Alexa, A., & Reményi, A. (2020). Co-regulation of the transcription controlling ATF2 phosphoswitch by JNK and p38. ''Nature Communications'', 11(1), 5769. https://doi.org/10.1038/s41467-020-19582-3
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Radhakrishnan, P., Basma, H., Klinkebiel, D., Christman, J., & Cheng, P.-W. (2008). Cell type-specific activation of the cytomegalovirus promoter by dimethylsulfoxide and 5-Aza-2’-deoxycytidine. ''The International Journal of Biochemistry & Cell Biology'', 40(9), 1944–1955. https://doi.org/10.1016/j.biocel.2008.02.014
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Zhang, C., Zhang, R., Dai, X., Cao, X., Wang, K., Huang, X., & Ren, Q. (2020). Activating transcription factor 2 (ATF2) negatively regulates the expression of antimicrobial peptide genes through tumor necrosis factor (TNF) in Macrobrachium nipponense. ''Fish & Shellfish Immunology'', 107, 26–35. https://doi.org/10.1016/j.fsi.2020.09.043
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Latest revision as of 16:55, 23 September 2024


CMV-ATF2-mRuby2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1398
    Illegal EcoRI site found at 1660
    Illegal XbaI site found at 1373
    Illegal XbaI site found at 1701
    Illegal PstI site found at 2108
    Illegal PstI site found at 2557
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1398
    Illegal EcoRI site found at 1660
    Illegal PstI site found at 2108
    Illegal PstI site found at 2557
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1398
    Illegal EcoRI site found at 1660
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1398
    Illegal EcoRI site found at 1660
    Illegal XbaI site found at 1373
    Illegal XbaI site found at 1701
    Illegal PstI site found at 2108
    Illegal PstI site found at 2557
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1398
    Illegal EcoRI site found at 1660
    Illegal XbaI site found at 1373
    Illegal XbaI site found at 1701
    Illegal PstI site found at 2108
    Illegal PstI site found at 2557
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 670
    Illegal SapI.rc site found at 2408


Design Notes

This composite part was codon optimised for human cell lines.


Source

The fluorescent marker mRuby was obtained from Eurofins and ATF2 was acquired from S. aureus.