Difference between revisions of "Part:BBa K5317017"
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===Usage and Biology===
 
===Usage and Biology===
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ATF2 belongs to the ATF/CREB family and regulates genes involved in cell growth, stress responses and apoptosis. With the addition of the ATF2 gene, this plasmid enables the study of transcriptional regulation of ATF2 (Kirsch et al., 2020) and its phosphorylation by PknB, making it important for research into signaling pathways related to cell stress and survival, (Zhang et al., 2020) while mRuby2 provides a fluorescent marker for visualization. In our cell-based β-lactam ring-containing antibiotics sensor, ATF2 serves as a translator of changes in PknB activity at the level of gene regulation, in particular the activity of the ATF2-3xCre2xAP1 promoter (BBa_K5317017).
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ATF2 belongs to the ATF/CREB family and regulates genes involved in cell growth, stress responses and apoptosis. With the addition of the ATF2 gene, this plasmid enables the study of transcriptional regulation of ATF2 (Kirsch et al., 2020) and its phosphorylation by PknB, making it important for research into signaling pathways related to cell stress and survival (Zhang et al., 2020). The consensus sequence for the CRE (cAMP-responsive elements) sequence that ATF2 binds to is typically represented as 5'-GTGACGT[AC][AG]-3'.

Revision as of 16:27, 23 September 2024

3xCre3xAP1-miniCMV Promoter

Usage and Biology

ATF2 belongs to the ATF/CREB family and regulates genes involved in cell growth, stress responses and apoptosis. With the addition of the ATF2 gene, this plasmid enables the study of transcriptional regulation of ATF2 (Kirsch et al., 2020) and its phosphorylation by PknB, making it important for research into signaling pathways related to cell stress and survival (Zhang et al., 2020). The consensus sequence for the CRE (cAMP-responsive elements) sequence that ATF2 binds to is typically represented as 5'-GTGACGT[AC][AG]-3'.