Difference between revisions of "Part:BBa K5299200"
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+ | ===References=== | ||
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+ | *[1]: Jaishankar, J., & Srivastava, P. (2020). Strong synthetic stationary phase promoter-based gene expression system for Escherichia coli. <i> Plasmid, 109 </i>(102491), 102491. doi:10.1016/j.plasmid.2020.102491 | ||
+ | *[2]: Pédelacq, J.-D., Cabantous, S., Tran, T., Terwilliger, T. C., & Waldo, G. S. (2006). Engineering and characterization of a superfolder green fluorescent protein. <i> Nature Biotechnology, 24 </i>(1), 79–88. doi:10.1038/nbt1172 |
Revision as of 11:41, 23 September 2024
P3.1 - BBa_B0030 - BBa_I746916 - BBa_B0015
It consists of a promoter[1], a RBS, a sfGFP[2] and a terminator.
It is able to produce the sfGFP according to the promoter's abilities.
It was constructed in order to check the strength of the P3.1 promoter. In the future, it can be used by other teams that wish to utilise the P3.1 promoter, in order to estimate that P3.1 is a suitable candidate in their design for their chassis of choice.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 259
References
- [1]: Jaishankar, J., & Srivastava, P. (2020). Strong synthetic stationary phase promoter-based gene expression system for Escherichia coli. Plasmid, 109 (102491), 102491. doi:10.1016/j.plasmid.2020.102491
- [2]: Pédelacq, J.-D., Cabantous, S., Tran, T., Terwilliger, T. C., & Waldo, G. S. (2006). Engineering and characterization of a superfolder green fluorescent protein. Nature Biotechnology, 24 (1), 79–88. doi:10.1038/nbt1172