Difference between revisions of "Part:BBa K257017:Design"

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===Design Notes===
 
===Design Notes===
 
Fec I needs to be expressed in association with Fec R behind the Pfec promoter in order to have a functional transduction pathway.
 
Fec I needs to be expressed in association with Fec R behind the Pfec promoter in order to have a functional transduction pathway.
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The prefix and suffix used to create this parts conform to the std 10 guidelines for coding DNA.
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This part is inserted in PSB1A3.
  
 
===Source===
 
===Source===
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===References===
 
===References===
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For more information, see: Gene regulation by transmembrane signaling. Volkmar Braun et al., 2006

Latest revision as of 16:10, 21 October 2009

Fec I, part of the signal transduction pathway FecARI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 335
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 338


Design Notes

Fec I needs to be expressed in association with Fec R behind the Pfec promoter in order to have a functional transduction pathway. The prefix and suffix used to create this parts conform to the std 10 guidelines for coding DNA. This part is inserted in PSB1A3.

Source

Sequence from E. coli k 12 strain MG1655 (refseq accessor NC_000913.2)

References

For more information, see: Gene regulation by transmembrane signaling. Volkmar Braun et al., 2006