Difference between revisions of "Part:BBa K5208005"

 
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AhlS encodes for an N-acyl homoserine lactonase in S. silvestris StLB214. This enzyme catalyzes the hydrolysis of N-Acyl homoserine lactone (AHL) (Morohoshi et al., 2012).
 
AhlS encodes for an N-acyl homoserine lactonase in S. silvestris StLB214. This enzyme catalyzes the hydrolysis of N-Acyl homoserine lactone (AHL) (Morohoshi et al., 2012).
  
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===Usage and Biology===
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<center><img src = "https://static.igem.wiki/teams/5208/parts/00-1.png" style = "width:600px"></center>
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<figcaption><center>Figure 1. Degradation of N-acyl homoserine lactone (AHL) by AhlS. The dashed line on the left shows where the enzyme breaks the bond (Chen et al., 2013; Dong et al., 2000).  </center></figcaption>
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
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<partinfo>BBa_K5208005 parameters</partinfo>
 
<partinfo>BBa_K5208005 parameters</partinfo>
 
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===Usage and Biology===
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AhlS degrades AHLs, a class of signaling molecules that induce quorum sensing. Quorum sensing triggers the expression of virulence factors in pathogens, including Aeromonas hydrophila, an aquatic bacterium that threatens aquaculture (Coquant et al., 2020; Hlordzi et al., 2020). High expression of AhlS in the probiotic Bacillus subtilis reduces the virulence of A. hydrophila through quorum quenching and saves aquatic animals.
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<figcaption><center>Figure 2. Gel electrophoresis of PCR product of AhlS. </center></figcaption>
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==References==
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Chen, F., Gao, Y., Chen, X., Yu, Z., & Li, X. (2013). Quorum quenching enzymes and their application in degrading signal molecules to block quorum sensing-dependent infection. Int J Mol Sci, 14(9), 17477-17500. https://doi.org/10.3390/ijms140917477
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Coquant, G., Grill, J. P., & Seksik, P. (2020). Impact of N-Acyl-Homoserine Lactones, Quorum Sensing Molecules, on Gut Immunity. Front Immunol, 11, 1827. https://doi.org/10.3389/fimmu.2020.01827
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Dong, Y. H., Xu, J. L., Li, X. Z., & Zhang, L. H. (2000). AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora. Proc Natl Acad Sci U S A, 97(7), 3526-3531. https://doi.org/10.1073/pnas.97.7.3526
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Hlordzi, V., Kuebutornye, F. K. A., Afriyie, G., Abarike, E. D., Lu, Y., Chi, S., & Anokyewaa, M. A. (2020). The use of Bacillus species in maintenance of water quality in aquaculture: A review. Aquaculture Reports, 18, 100503. https://doi.org/https://doi.org/10.1016/j.aqrep.2020.100503
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Morohoshi, T., Tominaga, Y., Someya, N., & Ikeda, T. (2012). Complete genome sequence and characterization of the N-acylhomoserine lactone-degrading gene of the potato leaf-associated Solibacillus silvestris. J Biosci Bioeng, 113(1), 20-25. https://doi.org/10.1016/j.jbiosc.2011.09.006

Revision as of 04:33, 23 September 2024


AhlS, N-acyl homoserine lactonase from Solibacillus silvestris StLB214

AhlS encodes for an N-acyl homoserine lactonase in S. silvestris StLB214. This enzyme catalyzes the hydrolysis of N-Acyl homoserine lactone (AHL) (Morohoshi et al., 2012).

Figure 1. Degradation of N-acyl homoserine lactone (AHL) by AhlS. The dashed line on the left shows where the enzyme breaks the bond (Chen et al., 2013; Dong et al., 2000).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

AhlS degrades AHLs, a class of signaling molecules that induce quorum sensing. Quorum sensing triggers the expression of virulence factors in pathogens, including Aeromonas hydrophila, an aquatic bacterium that threatens aquaculture (Coquant et al., 2020; Hlordzi et al., 2020). High expression of AhlS in the probiotic Bacillus subtilis reduces the virulence of A. hydrophila through quorum quenching and saves aquatic animals.

Figure 2. Gel electrophoresis of PCR product of AhlS.

References

Chen, F., Gao, Y., Chen, X., Yu, Z., & Li, X. (2013). Quorum quenching enzymes and their application in degrading signal molecules to block quorum sensing-dependent infection. Int J Mol Sci, 14(9), 17477-17500. https://doi.org/10.3390/ijms140917477

Coquant, G., Grill, J. P., & Seksik, P. (2020). Impact of N-Acyl-Homoserine Lactones, Quorum Sensing Molecules, on Gut Immunity. Front Immunol, 11, 1827. https://doi.org/10.3389/fimmu.2020.01827

Dong, Y. H., Xu, J. L., Li, X. Z., & Zhang, L. H. (2000). AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora. Proc Natl Acad Sci U S A, 97(7), 3526-3531. https://doi.org/10.1073/pnas.97.7.3526

Hlordzi, V., Kuebutornye, F. K. A., Afriyie, G., Abarike, E. D., Lu, Y., Chi, S., & Anokyewaa, M. A. (2020). The use of Bacillus species in maintenance of water quality in aquaculture: A review. Aquaculture Reports, 18, 100503. https://doi.org/https://doi.org/10.1016/j.aqrep.2020.100503

Morohoshi, T., Tominaga, Y., Someya, N., & Ikeda, T. (2012). Complete genome sequence and characterization of the N-acylhomoserine lactone-degrading gene of the potato leaf-associated Solibacillus silvestris. J Biosci Bioeng, 113(1), 20-25. https://doi.org/10.1016/j.jbiosc.2011.09.006