Difference between revisions of "Part:BBa K5208000"

 
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AiiA encodes for an N-acyl homoserine lactonase in B. subtilis BS-1. This enzyme catalyzes the hydrolysis of N-Acyl homoserine lactone (AHL) (Pan et al., 2008).
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This enzyme catalyzes the hydrolysis of N-Acyl homoserine lactone (AHL) (Pan et al., 2008).
  
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<center><img src = "https://static.igem.wiki/teams/4883/wiki/part-bba-k4883000-1.png" style = "width:480px"></center>
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<partinfo>BBa_K5208000 SequenceAndFeatures</partinfo>
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<figcaption><center>Figure 1. Degradation of N-acyl homoserine lactone (AHL) by AiiA. The dashed line on the left shows where the enzyme breaks the bond (Chen et al., 2013; Dong et al., 2000).  </center></figcaption>
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<partinfo>BBa_K5208000 parameters</partinfo>
 
<partinfo>BBa_K5208000 parameters</partinfo>
 
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<span class='h3bb'></span>
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===Sequence and Features===
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<partinfo>BBa_K5208000 SequenceAndFeatures</partinfo>
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<!-- Add more about the biology of this part here -->
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===Usage and Biology===
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AiiA degrades AHLs, a class of signaling molecules that induce quorum sensing. Quorum sensing triggers the expression of virulence factors in pathogens, including Aeromonas hydrophila, an aquatic bacterium that threatens aquaculture (Coquant et al., 2020; Hlordzi et al., 2020). High expression of AiiA in the probiotic B. subtilis reduces the virulence of A. hydrophila through quorum quenching and saves aquatic animals.
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<figure>
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<div class = "center">
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<center><img src = "https://static.igem.wiki/teams/4883/wiki/part-bba-k4883000-1.png" style = "width:480px"></center>
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<figcaption><center>Figure 2. Gel electrophoresis of PCR product of AiiA. </center></figcaption>
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==References==
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Chen, F., Gao, Y., Chen, X., Yu, Z., & Li, X. (2013). Quorum quenching enzymes and their application in degrading signal molecules to block quorum sensing-dependent infection. Int J Mol Sci, 14(9), 17477-17500. https://doi.org/10.3390/ijms140917477
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Coquant, G., Grill, J. P., & Seksik, P. (2020). Impact of N-Acyl-Homoserine Lactones, Quorum Sensing Molecules, on Gut Immunity. Front Immunol, 11, 1827. https://doi.org/10.3389/fimmu.2020.01827
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Dong, Y. H., Xu, J. L., Li, X. Z., & Zhang, L. H. (2000). AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora. Proc Natl Acad Sci U S A, 97(7), 3526-3531. https://doi.org/10.1073/pnas.97.7.3526
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Hlordzi, V., Kuebutornye, F. K. A., Afriyie, G., Abarike, E. D., Lu, Y., Chi, S., & Anokyewaa, M. A. (2020). The use of Bacillus species in maintenance of water quality in aquaculture: A review. Aquaculture Reports, 18, 100503. https://doi.org/https://doi.org/10.1016/j.aqrep.2020.100503
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Pan, J., Huang, T., Yao, F., Huang, Z., Powell, C. A., Qiu, S., & Guan, X. (2008). Expression and characterization of aiiA gene from Bacillus subtilis BS-1. Microbiol Res, 163(6), 711-716. https://doi.org/10.1016/j.micres.2007.12.002

Revision as of 03:31, 23 September 2024


AiiA, N-acyl homoserine lactonase from Bacillus subtilis BS-1

This enzyme catalyzes the hydrolysis of N-Acyl homoserine lactone (AHL) (Pan et al., 2008).

Figure 1. Degradation of N-acyl homoserine lactone (AHL) by AiiA. The dashed line on the left shows where the enzyme breaks the bond (Chen et al., 2013; Dong et al., 2000).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Usage and Biology

AiiA degrades AHLs, a class of signaling molecules that induce quorum sensing. Quorum sensing triggers the expression of virulence factors in pathogens, including Aeromonas hydrophila, an aquatic bacterium that threatens aquaculture (Coquant et al., 2020; Hlordzi et al., 2020). High expression of AiiA in the probiotic B. subtilis reduces the virulence of A. hydrophila through quorum quenching and saves aquatic animals.

Figure 2. Gel electrophoresis of PCR product of AiiA.


References

Chen, F., Gao, Y., Chen, X., Yu, Z., & Li, X. (2013). Quorum quenching enzymes and their application in degrading signal molecules to block quorum sensing-dependent infection. Int J Mol Sci, 14(9), 17477-17500. https://doi.org/10.3390/ijms140917477

Coquant, G., Grill, J. P., & Seksik, P. (2020). Impact of N-Acyl-Homoserine Lactones, Quorum Sensing Molecules, on Gut Immunity. Front Immunol, 11, 1827. https://doi.org/10.3389/fimmu.2020.01827

Dong, Y. H., Xu, J. L., Li, X. Z., & Zhang, L. H. (2000). AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora. Proc Natl Acad Sci U S A, 97(7), 3526-3531. https://doi.org/10.1073/pnas.97.7.3526

Hlordzi, V., Kuebutornye, F. K. A., Afriyie, G., Abarike, E. D., Lu, Y., Chi, S., & Anokyewaa, M. A. (2020). The use of Bacillus species in maintenance of water quality in aquaculture: A review. Aquaculture Reports, 18, 100503. https://doi.org/https://doi.org/10.1016/j.aqrep.2020.100503

Pan, J., Huang, T., Yao, F., Huang, Z., Powell, C. A., Qiu, S., & Guan, X. (2008). Expression and characterization of aiiA gene from Bacillus subtilis BS-1. Microbiol Res, 163(6), 711-716. https://doi.org/10.1016/j.micres.2007.12.002