Difference between revisions of "Part:BBa K257006:Design"
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# does not require any modification of the cleavage site during the secretion process, in contrast to the polipoprotein | # does not require any modification of the cleavage site during the secretion process, in contrast to the polipoprotein | ||
| − | C-term fusion using RFC23 ( | + | C-term fusion using RFC23 (silver). We can use this sequence signal with TolRII domain (fusion N term) or TE3 Fusion (Nterm) |
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| + | This part is Inserted in Topo Vector. | ||
===Source=== | ===Source=== | ||
Latest revision as of 15:51, 21 October 2009
ompA sequence signal
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Amplified from the secretion vector pIN-III-ompA-2 (Ghrayeb et al, 1984) keeping the RBS
- functions efficiently to secrete a large amount of the OmpA protein
- does not require any modification of the cleavage site during the secretion process, in contrast to the polipoprotein
C-term fusion using RFC23 (silver). We can use this sequence signal with TolRII domain (fusion N term) or TE3 Fusion (Nterm)
This part is Inserted in Topo Vector.
Source
pIN-III-ompA-2
References
Ghrayeb et al 1984 - "Secretion Cloning Vectors in Escherichia coli" The EMBO Journal 3(10):2437-2442