Difference between revisions of "Part:BBa K5246003"

 
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<partinfo>BBa_K5246003 short</partinfo>
 
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Gene from Caulobacter crescentud HfsC, encodes a protein of 422aa that polymerases repeats of monomers into a mature holdfast polymer, deletion of polysaccharide polymerase gene hfsC in C.Crescentus didn't cause holdfast synthesis defects, because of its paralogue - HfsI. Double mutants of HfsC and HfsI cause severe holfast synthesis defects
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===Introduction===
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===Usage and Biology===
 
===Usage and Biology===
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Gene from Caulobacter crescentud HfsC, encodes a protein of 422aa that polymerases repeats of monomers into a mature holdfast polymer, deletion of polysaccharide polymerase gene hfsC in C.Crescentus didn't cause holdfast synthesis defects, because of its paralogue - HfsI. Double mutants of HfsC and HfsI cause severe holfast synthesis defects
  
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===Sequence and Features===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K5246003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5246003 SequenceAndFeatures</partinfo>
  
  
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K5246003 parameters</partinfo>
 
<partinfo>BBa_K5246003 parameters</partinfo>
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===Experimental characterization===
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===References===

Revision as of 12:10, 22 September 2024


CB2/CB2A HfsC Polysaccharide polymerase

Introduction

Usage and Biology

Gene from Caulobacter crescentud HfsC, encodes a protein of 422aa that polymerases repeats of monomers into a mature holdfast polymer, deletion of polysaccharide polymerase gene hfsC in C.Crescentus didn't cause holdfast synthesis defects, because of its paralogue - HfsI. Double mutants of HfsC and HfsI cause severe holfast synthesis defects

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1072
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1072
    Illegal NotI site found at 745
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 491
    Illegal BglII site found at 863
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1072
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1072
    Illegal NgoMIV site found at 12
    Illegal NgoMIV site found at 109
    Illegal NgoMIV site found at 732
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

Experimental characterization

References