Difference between revisions of "Part:BBa K5036040"

 
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==Part Description==
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CRISPER multiplexing system is a versatile genetic engineering tool, empowers scientists to simultaneously target and modify multiple genes within an organism. By designing multiple guide RNAs that precisely bind to specific DNA sequences of interest, researchers can efficiently and accurately introduce desired genetic changes. This capability enhances efficiency and precision, making CRISPR a valuable asset for a wide range of biological applications.
  
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It is a versatile genetic engineering tool, empowers scientists to simultaneously target and modify multiple genes within an organism. By designing multiple guide RNAs that precisely bind to specific DNA sequences of interest, researchers can efficiently and accurately introduce desired genetic changes. This capability enhances efficiency and precision, making CRISPR a valuable asset for a wide range of biological applications
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==Usage==
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Our CRISPR multiplexing system consists of three components: a guide RNA targeting the Nanog gene to activate endogenous YAP production by (our dCAS9 transcription activator) complex, a guide RNA targeting a sequence to activate the production of mRNA containing the TID switch by (our dCAS9 transcription activator) complex, and the HHR enzyme which mediates self-cleavage to separate these guide RNAs into individual functional units.
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<html><div align="center"style="border:solid #17252A; width:100%;float:center;"><img style="                              max-width:850px;
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width:75%;
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height:auto;
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position: relative;
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top: 50%;
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left: 35%;
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transform: translate( -50%);
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padding-bottom:25px;
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"src="https://static.igem.wiki/teams/5036/parts/grna-multiplexing.png">
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<p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span
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lang=EN style='font-size:11.0pt;line-height:115%'>this figure illustrates the process our CRISPER multiplexing system which Allow us to fully utilize the capabilities of our dCas9(N/C) Syn-RTK system.
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.  </span></p></div></html>
  
 
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Revision as of 11:35, 22 September 2024


CRISPR multiplexing system (Nanog gRNA-HHR-gRNA operator)

Part Description

CRISPER multiplexing system is a versatile genetic engineering tool, empowers scientists to simultaneously target and modify multiple genes within an organism. By designing multiple guide RNAs that precisely bind to specific DNA sequences of interest, researchers can efficiently and accurately introduce desired genetic changes. This capability enhances efficiency and precision, making CRISPR a valuable asset for a wide range of biological applications.

Usage

Our CRISPR multiplexing system consists of three components: a guide RNA targeting the Nanog gene to activate endogenous YAP production by (our dCAS9 transcription activator) complex, a guide RNA targeting a sequence to activate the production of mRNA containing the TID switch by (our dCAS9 transcription activator) complex, and the HHR enzyme which mediates self-cleavage to separate these guide RNAs into individual functional units.

this figure illustrates the process our CRISPER multiplexing system which Allow us to fully utilize the capabilities of our dCas9(N/C) Syn-RTK system. .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 316
  • 1000
    COMPATIBLE WITH RFC[1000]