Difference between revisions of "Part:BBa K5036034"

(→‎Usage)
(→‎Usage)
Line 17: Line 17:
 
padding-bottom:25px;
 
padding-bottom:25px;
 
padding-top:25px;
 
padding-top:25px;
−
"src="https://static.igem.wiki/teams/5036/parts/vegf-r2.png">
+
"src="https://static.igem.wiki/teams/5036/parts/vegfr2-tev.png">
 
<p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span
 
<p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span
−
lang=EN style='font-size:11.0pt;line-height:115%'>This figure illustrates our receptor's second chain structure. </span></p></div></html>
+
lang=EN style='font-size:11.0pt;line-height:115%'>This figure illustrates variant of our receptor's second chain where TCS (Q, Q) is to it.
 +
 
 +
. </span></p></div></html>
  
  

Revision as of 18:25, 21 September 2024


dCas9(N)_NES-Syn-VEGFR-2 (VEGF-R2, C-TEV, NES, TCS (Q, L), HA, dCas9(N),mCherry)

Part Description

In our second receptor chain, we've engineered a system that responds to tissue injury. An external domain, VEGF-R2, is attached to an internal domain composed of C terminal domain of TEV protease, a nuclear export signal (NES), a TEV cleavage site(TCS(Q,L)), and dCas9(N).

Usage

this is our receptor's second chain. our receptor is activated after binding of VEGF to the external domain which is designed to attach specifically to it. after activation the two domains of TEV dimerizes forming catalytically active TEV protease which will cleave the two chains at TCS. upon cleavage of the two chains the two domains of dCas9 dimerize and is released attached to transcription activator to be guided to its direction

This figure illustrates variant of our receptor's second chain where TCS (Q, Q) is to it. .


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3352
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 982
    Illegal BglII site found at 2341
    Illegal BglII site found at 3662
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4489
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1854
    Illegal BsaI.rc site found at 664
    Illegal BsaI.rc site found at 1442
    Illegal BsaI.rc site found at 2731
    Illegal SapI.rc site found at 3283