Difference between revisions of "Part:BBa K5036029"
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this is our receptor's first chain. our receptor is activated after binding of VEGF to the external domain which is designed to attach specifically to it. after activation the two domains of TEV dimerizes forming catalytically active TEV protease which will cleave the two chains at TCS. upon cleavage of the two chains the two domains of dCas9 dimerize and is released attached to transcription activator to be guided to its direction | this is our receptor's first chain. our receptor is activated after binding of VEGF to the external domain which is designed to attach specifically to it. after activation the two domains of TEV dimerizes forming catalytically active TEV protease which will cleave the two chains at TCS. upon cleavage of the two chains the two domains of dCas9 dimerize and is released attached to transcription activator to be guided to its direction | ||
− | + | <html><div align="center"style="border:solid #17252A; width:100%;float:center;"><img style=" max-width:850px; | |
+ | width:70%; | ||
+ | height:auto; | ||
+ | position: relative; | ||
+ | top: 50%; | ||
+ | left: 35%; | ||
+ | transform: translate( -50%); | ||
+ | padding-bottom:25px; | ||
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+ | "src="https://static.igem.wiki/teams/5036/parts/vegfr1-tcs.png"> | ||
+ | <p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span | ||
+ | lang=EN style='font-size:11.0pt;line-height:115%'>This figure illustrates variant of our receptor's first chain where TCS (Q, L) is to it. </span></p></div></html> | ||
Revision as of 18:20, 21 September 2024
dCas9(C)_NLS-Syn-VEGFR-1 (VEGF-R1, C-TEV, NLS, TCS(Q,L), HA,dCas9(C),VP64,GFP)
Part Description
In our first receptor chain, we've engineered a system that responds to tissue injury. An external domain, VEGF-R1, is attached to an internal domain composed of C terminal domain of TEV protease, a nuclear localization signal (NLS), a TEV cleavage site(TCS(Q,L)), and dCas9(C) which is linked to transcription activator VP64
Usage
this is our receptor's first chain. our receptor is activated after binding of VEGF to the external domain which is designed to attach specifically to it. after activation the two domains of TEV dimerizes forming catalytically active TEV protease which will cleave the two chains at TCS. upon cleavage of the two chains the two domains of dCas9 dimerize and is released attached to transcription activator to be guided to its direction
This figure illustrates variant of our receptor's first chain where TCS (Q, L) is to it.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3973
Illegal NgoMIV site found at 4046
Illegal NgoMIV site found at 4531
Illegal NgoMIV site found at 5440 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 6737
Illegal SapI.rc site found at 3268