Difference between revisions of "Part:BBa K5061011"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | Protein III is the minor coat protein (CoaA) of the M13 bacteriophage [Knezevic et al., 2021]. It is located on the end of the M13 filament | + | Protein III is the minor coat protein (CoaA) of the M13 bacteriophage [Knezevic et al., 2021]. It is located on the end of the M13 filament alongside the protein VI, while at the oposite end are located proteins VII and VIII (Figure 1). |
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Figure 1. Schematic representation of the M13 virion. | Figure 1. Schematic representation of the M13 virion. | ||
| − | + | The C-terminus of pritein III is involved in terminating phage assembly, while its N-terminus binds to the bacterial pili to initiate infection. | |
| − | + | ||
The mutation G378S is present in the VCSM13 helper phage (GenBank AY598820), in the M13KO7 helper phage we recovered from the tube provided by New England Biolabs ([[Part:BBa_K5061000|BBa_K5061000]]), but not in the M13 Phage (GenBank V00604). | The mutation G378S is present in the VCSM13 helper phage (GenBank AY598820), in the M13KO7 helper phage we recovered from the tube provided by New England Biolabs ([[Part:BBa_K5061000|BBa_K5061000]]), but not in the M13 Phage (GenBank V00604). | ||
Revision as of 13:42, 21 September 2024
M13 bacteriophage gIII G378S
This part is the protein III of the M13 bacteriophage with one mutation: G378S.
Usage and Biology
Protein III is the minor coat protein (CoaA) of the M13 bacteriophage [Knezevic et al., 2021]. It is located on the end of the M13 filament alongside the protein VI, while at the oposite end are located proteins VII and VIII (Figure 1).
Figure 1. Schematic representation of the M13 virion.
The C-terminus of pritein III is involved in terminating phage assembly, while its N-terminus binds to the bacterial pili to initiate infection.
The mutation G378S is present in the VCSM13 helper phage (GenBank AY598820), in the M13KO7 helper phage we recovered from the tube provided by New England Biolabs (BBa_K5061000), but not in the M13 Phage (GenBank V00604).
References
Knezevic P, Adriaenssens EM, Ictv Report Consortium. ICTV Virus Taxonomy Profile: Inoviridae. J Gen Virol. 2021 Jul;102(7):001614.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 642
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]