Difference between revisions of "Part:BBa K177002"

Latest revision as of 15:03, 21 October 2009

cro binding box

Cro-box is the particular fragment of phage lambda DNA which is recognized by cro repressor protein. This sequence is artificially designed (based on natural fragment of lambda phage genome) to increase the affinity for cro protein.

Usage and Biology

When this part is introduced into a sequence of elements expressed from a single promoter the binding of the Cro protein (BBa_K177009) successfully inhibits the expression of elements located downstream.

An alternative version of this sequence has also been created (BBa_K177047) which differs from this one by the binding strength of repressor proteins, Cro (BBa_K177009) and cI (BBa_C0051; BBa_K177050).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K177002 short</partinfo>
-derivative of OR1
+Cro-box is the particular fragment of phage lambda DNA which is recognized by cro repressor protein. This sequence is artificially designed (based on natural fragment of lambda phage genome) to increase the affinity for cro protein.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+When this part is introduced into a sequence of elements expressed from a single promoter the binding of the Cro protein (<partinfo>K177009</partinfo>) successfully inhibits the expression of elements located downstream.
+An alternative version of this sequence has also been created (<partinfo>K177047</partinfo>) which differs from this one by the binding strength of repressor proteins, Cro (<partinfo>K177009</partinfo>) and cI (<partinfo>C0051</partinfo>; <partinfo>K177050</partinfo>).
-<!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K177002 SequenceAndFeatures</partinfo>