Difference between revisions of "Part:BBa K5061006"

 
 
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<partinfo>BBa_K5061006 short</partinfo>
 
<partinfo>BBa_K5061006 short</partinfo>
  
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Golden Gate adapter for Accessory Plasmids (AP) Golden Gate cloning platforms for M13 gVI expression
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This Golden Gate adapter contains two BsaI recognition sites in opposite direction.
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It is present in our composite parts [[Part:BBa_K5061032|BBa_K5061032]] and [[Part:BBa_K5061132|BBa_K5061132]] and allows rapid engineering using Golden Gate Assembly of Accessory Plasmids (AP) for phage-assisted continuous and non-continuous evolution (PACE and PANCE).
  
 
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Latest revision as of 22:13, 20 September 2024


Golden Gate adapter for Accessory Plasmids (AP) Golden Gate cloning platforms for M13 gVI expression

This Golden Gate adapter contains two BsaI recognition sites in opposite direction.

It is present in our composite parts BBa_K5061032 and BBa_K5061132 and allows rapid engineering using Golden Gate Assembly of Accessory Plasmids (AP) for phage-assisted continuous and non-continuous evolution (PACE and PANCE).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 26
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 18
    Illegal BsaI.rc site found at 6