Difference between revisions of "Part:BBa K5396006"
(→‎Usage and Biology)
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__NOTOC__
 
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<partinfo>BBa_K5396010 short</partinfo>
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<partinfo>BBa_K5396006 short</partinfo>
  
 
This part is the N-terminal of Spidroin fused with our BARBIE1 protein with an additional cysteine (BBa_K5396004).  
 
This part is the N-terminal of Spidroin fused with our BARBIE1 protein with an additional cysteine (BBa_K5396004).  
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===Usage and Biology===
 
===Usage and Biology===
  
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<p>Spidroins are the primary proteins that compose spider silk. This part contains the N-terminal domain, which s involved in the initial formation of silk fibers and is crucial for the protein's solubility and stability.
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<p>Spidroins are the primary proteins that compose spider silk. This part contains the N-terminal domain, which s involved in the initial formation of silk fibers and is crucial for the protein's solubility and stability, and is fused to our BARBIE1 protein, that has the increased ability to bind to plastics.
  
 
===Part Generation===
 
===Part Generation===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K5396010 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K5396006 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K5396010 parameters</partinfo>
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<partinfo>BBa_K5396006 parameters</partinfo>
 
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Revision as of 16:44, 20 September 2024


Nt-Barbie1-Cys

This part is the N-terminal of Spidroin fused with our BARBIE1 protein with an additional cysteine (BBa_K5396004).

Usage and Biology

Spidroins are the primary proteins that compose spider silk. This part contains the N-terminal domain, which s involved in the initial formation of silk fibers and is crucial for the protein's solubility and stability, and is fused to our BARBIE1 protein, that has the increased ability to bind to plastics.

Part Generation

Gibson Assembly

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 490
  • 1000
    COMPATIBLE WITH RFC[1000]