Difference between revisions of "Part:BBa K5396006"

 
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<partinfo>BBa_K5396010 short</partinfo>
 
<partinfo>BBa_K5396010 short</partinfo>
  
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This part is the N-terminal of Spidroin fused with our Barbie protein with an additional cysteine (BBa_).  
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This part is the N-terminal of Spidroin fused with our BARBIE1 protein with an additional cysteine (BBa_K5396004).  
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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texto sobre a spidroina e barbie
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===Part Generation===
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Gibson Assembly
  
 
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Revision as of 16:28, 20 September 2024


T7-Nt-BaCBM2-Cys

This part is the N-terminal of Spidroin fused with our BARBIE1 protein with an additional cysteine (BBa_K5396004).

Usage and Biology

texto sobre a spidroina e barbie

Part Generation

Gibson Assembly

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 96
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 30
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 96
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 96
  • 1000
    COMPATIBLE WITH RFC[1000]