Difference between revisions of "Part:BBa K257018:Design"
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===Design Notes=== | ===Design Notes=== | ||
We had to deal with considerations about the linker domain which links the translocated protein to the translocator domain of AIDA-I. In fact, this linker needs to have a minimal length so that the protein translocated would be really functional at the outer side of the outer membrane. | We had to deal with considerations about the linker domain which links the translocated protein to the translocator domain of AIDA-I. In fact, this linker needs to have a minimal length so that the protein translocated would be really functional at the outer side of the outer membrane. | ||
| − | + | AIDA Translocator domain has been designed using a fusion silver standard 23 prefix in order to be able to make a fusion protein with the N terminal extremity of AIDA translocator. | |
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===Source=== | ===Source=== | ||
Revision as of 14:28, 21 October 2009
AIDA-I autotransporter, linker + translocator domains
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1118
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We had to deal with considerations about the linker domain which links the translocated protein to the translocator domain of AIDA-I. In fact, this linker needs to have a minimal length so that the protein translocated would be really functional at the outer side of the outer membrane. AIDA Translocator domain has been designed using a fusion silver standard 23 prefix in order to be able to make a fusion protein with the N terminal extremity of AIDA translocator.
Source
AIDA has been synthesized in vitro.