Difference between revisions of "Part:BBa K5492732:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | ssDNA sequence designed to be complementary for the 5' end of [https://parts.igem.org/Part:BBa_K5492722 s1_aptamer_with_fluorophore], it contains a quencher molecule which absorbs the light emitted by fluorophore at its 3' end, until the aptamer binds histamine. | |
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===Source=== | ===Source=== | ||
Latest revision as of 10:49, 19 September 2024
S1_aptamer_reverse_complement_strand
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
ssDNA sequence designed to be complementary for the 5' end of s1_aptamer_with_fluorophore, it contains a quencher molecule which absorbs the light emitted by fluorophore at its 3' end, until the aptamer binds histamine.
Source
https://pubs.acs.org/doi/abs/10.1021/acs.analchem.9b00075 Reference: Zhang, H.; Lai, Y.; Wang, L.; Chen, Z.; Wang, Z. High-Throughput Screening of DNA Aptamers for Bisphenol A Detection Based on Circular Dichroism and Fluorescence. Anal. Chem. 2019, 91 (10), 6581–6588. DOI: 10.1021/acs.analchem.9b00075.
References
Reference: Zhang, H.; Lai, Y.; Wang, L.; Chen, Z.; Wang, Z. High-Throughput Screening of DNA Aptamers for Bisphenol A Detection Based on Circular Dichroism and Fluorescence. Anal. Chem. 2019, 91 (10), 6581–6588. DOI: 10.1021/acs.analchem.9b00075.