Difference between revisions of "Part:BBa K5492732:Design"

 
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__NOTOC__
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<partinfo>BBa_K5492732 short</partinfo>
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<partinfo>BBa_K5492732 SequenceAndFeatures</partinfo>
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===Design Notes===
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ssDNA sequence designed to be complementary for the 5' end of [https://parts.igem.org/Part:BBa_K5492722 s1_aptamer_with_fluorophore], it contains a quencher molecule which absorbs the light emitted by fluorophore at its 3' end, until the aptamer binds histamine.
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===Source===
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https://pubs.acs.org/doi/abs/10.1021/acs.analchem.9b00075 Reference: Zhang, H.; Lai, Y.; Wang, L.; Chen, Z.; Wang, Z. High-Throughput Screening of DNA Aptamers for Bisphenol A Detection Based on Circular Dichroism and Fluorescence. Anal. Chem. 2019, 91 (10), 6581–6588. DOI: 10.1021/acs.analchem.9b00075.
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===References===
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Reference: Zhang, H.; Lai, Y.; Wang, L.; Chen, Z.; Wang, Z. High-Throughput Screening of DNA Aptamers for Bisphenol A Detection Based on Circular Dichroism and Fluorescence. Anal. Chem. 2019, 91 (10), 6581–6588. DOI: 10.1021/acs.analchem.9b00075.

Latest revision as of 10:49, 19 September 2024


S1_aptamer_reverse_complement_strand


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ssDNA sequence designed to be complementary for the 5' end of s1_aptamer_with_fluorophore, it contains a quencher molecule which absorbs the light emitted by fluorophore at its 3' end, until the aptamer binds histamine.

Source

https://pubs.acs.org/doi/abs/10.1021/acs.analchem.9b00075 Reference: Zhang, H.; Lai, Y.; Wang, L.; Chen, Z.; Wang, Z. High-Throughput Screening of DNA Aptamers for Bisphenol A Detection Based on Circular Dichroism and Fluorescence. Anal. Chem. 2019, 91 (10), 6581–6588. DOI: 10.1021/acs.analchem.9b00075.

References

Reference: Zhang, H.; Lai, Y.; Wang, L.; Chen, Z.; Wang, Z. High-Throughput Screening of DNA Aptamers for Bisphenol A Detection Based on Circular Dichroism and Fluorescence. Anal. Chem. 2019, 91 (10), 6581–6588. DOI: 10.1021/acs.analchem.9b00075.