Difference between revisions of "Part:BBa K5492721:Design"
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[https://parts.igem.org/Part:BBa_K5492711 J1 ssDNA aptamer] sequence designed to specifically bind to histamine. | [https://parts.igem.org/Part:BBa_K5492711 J1 ssDNA aptamer] sequence designed to specifically bind to histamine. | ||
The sequence contains a fluorophore molecule at its 5' end. | The sequence contains a fluorophore molecule at its 5' end. | ||
| − | [https://parts.igem.org/Part:BBa_K5492731 j1_reverse_compliment_strand] is complementary to this sequnce, which contains a quencher molecule which absorbs the light emitted by | + | [https://parts.igem.org/Part:BBa_K5492731 j1_reverse_compliment_strand] is complementary to this sequnce, which contains a quencher molecule which absorbs the light emitted by fluorophore until the aptamer binds histamine. |
===Source=== | ===Source=== | ||
Revision as of 10:33, 19 September 2024
J1_aptamer_with_fluorophore
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
J1 ssDNA aptamer sequence designed to specifically bind to histamine. The sequence contains a fluorophore molecule at its 5' end. j1_reverse_compliment_strand is complementary to this sequnce, which contains a quencher molecule which absorbs the light emitted by fluorophore until the aptamer binds histamine.
Source
https://www.sciencedirect.com/science/article/abs/pii/S0039914019311075?via%3Dihub Reference: Zhao, Q.; Fan, T.; Chen, L.; Fu, B.; Zhou, Y.; Zheng, X.; Wu, J. Development of Aptamers for Recognition and Separation of Toxins. Talanta 2020, 211, 120725. DOI: 10.1016/j.talanta.2020.120725.
References
Reference: Zhao, Q.; Fan, T.; Chen, L.; Fu, B.; Zhou, Y.; Zheng, X.; Wu, J. Development of Aptamers for Recognition and Separation of Toxins. Talanta 2020, 211, 120725. DOI: 10.1016/j.talanta.2020.120725.