Difference between revisions of "Part:BBa K5492702:Design"

(→‎Source)
(→‎Design Notes)
 
(5 intermediate revisions by one other user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
a biotin molecule is attached to it in the final part and
+
5' biotin tag attached was attached to the sequence.
−
part of it is complementary to BBa_K5492712 (H5_Sulivan_to_BEAD)
+
The 3' part of the sequence is complementary to our [https://parts.igem.org/Part:BBa_K5492712 S1_aptamer] part.
−
 
+
−
 
+
−
 
+
−
 
+
  
 
===Source===
 
===Source===
−
 
+
https://pubs.acs.org/doi/abs/10.1021/acs.analchem.9b00075
−
Reference: Zhao, Q.; Fan, T.; Chen, L.; Fu, B.; Zhou, Y.; Zheng, X.; Wu, J. Development of Aptamers for Recognition and Separation of Toxins. Talanta 2020, 211, 120725. DOI: 10.1016/j.talanta.2020.120725.
+
Reference: Zhang, H.; Lai, Y.; Wang, L.; Chen, Z.; Wang, Z. High-Throughput Screening of DNA Aptamers for Bisphenol A Detection Based on Circular Dichroism and Fluorescence. Anal. Chem. 2019, 91 (10), 6581–6588. DOI: 10.1021/acs.analchem.9b00075.
  
 
===References===
 
===References===
 +
Reference: Zhang, H.; Lai, Y.; Wang, L.; Chen, Z.; Wang, Z. High-Throughput Screening of DNA Aptamers for Bisphenol A Detection Based on Circular Dichroism and Fluorescence. Anal. Chem. 2019, 91 (10), 6581–6588. DOI: 10.1021/acs.analchem.9b00075.

Latest revision as of 09:48, 19 September 2024


S1_aptamer_connector


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

5' biotin tag attached was attached to the sequence. The 3' part of the sequence is complementary to our S1_aptamer part.

Source

https://pubs.acs.org/doi/abs/10.1021/acs.analchem.9b00075 Reference: Zhang, H.; Lai, Y.; Wang, L.; Chen, Z.; Wang, Z. High-Throughput Screening of DNA Aptamers for Bisphenol A Detection Based on Circular Dichroism and Fluorescence. Anal. Chem. 2019, 91 (10), 6581–6588. DOI: 10.1021/acs.analchem.9b00075.

References

Reference: Zhang, H.; Lai, Y.; Wang, L.; Chen, Z.; Wang, Z. High-Throughput Screening of DNA Aptamers for Bisphenol A Detection Based on Circular Dichroism and Fluorescence. Anal. Chem. 2019, 91 (10), 6581–6588. DOI: 10.1021/acs.analchem.9b00075.