Difference between revisions of "Part:BBa K5374010:Design"

 
(→‎Design Notes)
 
(One intermediate revision by the same user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
None
+
The SPARC/OD sequence was optimized for fusion with EGFP to maintain collagen-binding activity without disrupting EGFP fluorescence, providing insight into the impact of SPARC as a release tag for cell factors.
−
 
+
−
 
+
  
 
===Source===
 
===Source===
  
−
EGFP = Enhanced green fluorescent protein which can be used as a reporter gene.
+
The SPARC domain is derived from the human SPARC gene. EGFP is sourced from BBa_K1911005, a widely used fluorescence marker.
  
 
===References===
 
===References===

Latest revision as of 09:10, 19 September 2024


SPARC/OD-EGFP. A collagen-binding domain involved in tissue repair and bone mineralization, fused wi


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 484
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 484
    Illegal NheI site found at 922
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 484
    Illegal BglII site found at 897
    Illegal BamHI site found at 288
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 484
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 484
    Illegal NgoMIV site found at 859
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1565


Design Notes

The SPARC/OD sequence was optimized for fusion with EGFP to maintain collagen-binding activity without disrupting EGFP fluorescence, providing insight into the impact of SPARC as a release tag for cell factors.

Source

The SPARC domain is derived from the human SPARC gene. EGFP is sourced from BBa_K1911005, a widely used fluorescence marker.

References