Difference between revisions of "Part:BBa K201007:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This part was designed with the same structure of the Berkley constitutive promoters. Indeed, the operator sequence was digested with SpeI and PstI and the same was done for the RFP. The two parts were then ligated. As a second passage, the obtained O2-RFP part was digested with XbaI and PstI and ligated downstream of the BBa_J23118 promoter digested with SpeI and PstI.This was done to allow an easier cloning of the operator and promoter sequences that are short in length (about 20 bp and 35 bp, respectively). | + | This part was designed with the same structure of the [https://parts.igem.org/Part:BBa_J23100 Berkley constitutive promoters]. Indeed, the operator sequence was digested with SpeI and PstI and the same was done for the RFP. The two parts were then ligated. As a second passage, the obtained O2-RFP part was digested with XbaI and PstI and ligated downstream of the BBa_J23118 promoter digested with SpeI and PstI.This was done to allow an easier cloning of the operator and promoter sequences that are short in length (about 20 bp and 35 bp, respectively). |
===Source=== | ===Source=== | ||
Latest revision as of 14:06, 21 October 2009
LacI repressed BBa_J23118 promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 625
Illegal AgeI site found at 737 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was designed with the same structure of the Berkley constitutive promoters. Indeed, the operator sequence was digested with SpeI and PstI and the same was done for the RFP. The two parts were then ligated. As a second passage, the obtained O2-RFP part was digested with XbaI and PstI and ligated downstream of the BBa_J23118 promoter digested with SpeI and PstI.This was done to allow an easier cloning of the operator and promoter sequences that are short in length (about 20 bp and 35 bp, respectively).
Source
All subparts come from the Registry of Standard Parts