Difference between revisions of "Part:BBa K216009:Experience"
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This part was designed to characterize the activity of the PyeaR promoter by Miller assay, as a backup to the GFP-based construct used in the promoter characterization kit. | This part was designed to characterize the activity of the PyeaR promoter by Miller assay, as a backup to the GFP-based construct used in the promoter characterization kit. | ||
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| + | <b><u>Results from Miller's Assay</b></u> | ||
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| + | [[Image:PyeaR-miller.png]] | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 13:47, 21 October 2009
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Applications of BBa_K216009
This part was designed to characterize the activity of the PyeaR promoter by Miller assay, as a backup to the GFP-based construct used in the promoter characterization kit.
Results from Miller's Assay</b>
User Reviews
Initial Tests: Edinburgh iGEM 2009: To test this part, we grew E. coli JM109 overnight at 37 C with shaking in LB with 80 mg/l ampicillin and varying concentrations of sodium nitrate and sodium nitrite. The following day, we performed Miller assays (LacZ activity assays using o-nitrophenyl galactoside, ONPG, as a chromogenic substrate). The results showed strong induction by both nitrate and nitrite, as expected for this promoter (see biology information for part BBa_K216005).
UNIQ47adfda84ecbc650-partinfo-00000000-QINU UNIQ47adfda84ecbc650-partinfo-00000001-QINU