Difference between revisions of "Part:BBa K216005:Experience"
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Edinburgh iGEM team, October 2009: To test this part, it was combined with lacZ minigene J33202 for Miller assays (giving part BBa_K216009), and also with GFP for analysis using the promoter characterization kit of Jason Kelly. Results from overnight induction with Miller assay indicated stronger induction by nitrite than nitrate, but a time course of induction using the GFP construct indicated that nitrate gave stronger induction in the first few hours. | Edinburgh iGEM team, October 2009: To test this part, it was combined with lacZ minigene J33202 for Miller assays (giving part BBa_K216009), and also with GFP for analysis using the promoter characterization kit of Jason Kelly. Results from overnight induction with Miller assay indicated stronger induction by nitrite than nitrate, but a time course of induction using the GFP construct indicated that nitrate gave stronger induction in the first few hours. | ||
<p> | <p> | ||
| − | <b>Results from Miller's Assay</b> | + | <b><u>Results from Miller's Assay</u></b> |
<br> | <br> | ||
[[Image:PyeaR-miller.png]] | [[Image:PyeaR-miller.png]] | ||
| − | < | + | <p><p> |
| − | <b>Results from GFP promoter characterization kit.</b> | + | <b><u>Results from GFP promoter characterization kit.</u></b> |
<br> | <br> | ||
[[Image:Pyear_characterization.jpg]] | [[Image:Pyear_characterization.jpg]] | ||
Revision as of 13:45, 21 October 2009
This experience page is provided so that any user may enter their experience using this part.
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Applications of BBa_K216005
Edinburgh iGEM team, October 2009: To test this part, it was combined with lacZ minigene J33202 for Miller assays (giving part BBa_K216009), and also with GFP for analysis using the promoter characterization kit of Jason Kelly. Results from overnight induction with Miller assay indicated stronger induction by nitrite than nitrate, but a time course of induction using the GFP construct indicated that nitrate gave stronger induction in the first few hours.
Results from Miller's Assay
<p><p>
Results from GFP promoter characterization kit.
User Reviews
UNIQ07873b28aca4fc40-partinfo-00000000-QINU
UNIQ07873b28aca4fc40-partinfo-00000001-QINU