Difference between revisions of "Part:BBa K243024"
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This combination of parts use the benefits of an Strep-tag for purification. It is also linked with a FluroescineA tag. The Middle Linker (GlySerGlyGly)x2 connects the parts and adds additional space between them to guarantee       
 
This combination of parts use the benefits of an Strep-tag for purification. It is also linked with a FluroescineA tag. The Middle Linker (GlySerGlyGly)x2 connects the parts and adds additional space between them to guarantee       
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the independent function of FluA tag and the protein domain Fok_a.The [https://parts.igem.org/wiki/index.php?title=Part:BBa_K157004 fluoresceinA tag] allows the measurement by [quenching] and the coupling to an flurescein linked oligo.<br>
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the independent function of FluA tag and the protein domain Fok_a.<br>
  
  
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
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We used this combination , which allows the measurement by quenching and the coupling to an flurescein linked oligo
  
 
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Revision as of 13:42, 21 October 2009

Strep-FluA-Middle Linker-Fok_a

This combination of parts use the benefits of an Strep-tag for purification. It is also linked with a FluroescineA tag. The Middle Linker (GlySerGlyGly)x2 connects the parts and adds additional space between them to guarantee the independent function of FluA tag and the protein domain Fok_a.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1075