Difference between revisions of "Part:BBa K5136026"
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<b>Cellulase EG5C</b>
 
<b>Cellulase EG5C</b>
 
<br>
 
<br>
 +
<b>Biology</b>
 +
<br>
 +
<b>Cellulase</b>
 +
<br>
 +
Efficient hydrolysis of cellulose to glucose depends on the synchronized action of three classes of enzymes, endoglucanase, exoglucanase and β-glucosidase. Processivity is a common mode of action for many exoglucanases and plays a key role in the complete hydrolysis of crystalline cellulose. In contrast, classic endoglucanases randomly cleave β-1,4-glycosidic bonds in the interior of the cellulosic chains and have been considered to be non-processive. Studies have shown that in contrast to other cellulolytic systems that dependent on both endo and exoglucanases, a processive endoglucanase coupled with a β-glucosidase may be sufficient for the degradation of cellulose. Cellulase EG5C is a kind of proteins found from
 +
<b><i>Bacillus subtilis</I></b>
 +
BS-5 (1). It is hypothesized that
 +
<b><i>B. subtilis</I></b>
 +
BS-5 might produce a processive endoglucanase, which might substitute for the apparent deficiency in exoglucanase activity.
  
 
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Revision as of 12:42, 17 September 2024


cellulase EG5C-His tag


Cellulase EG5C
Biology
Cellulase
Efficient hydrolysis of cellulose to glucose depends on the synchronized action of three classes of enzymes, endoglucanase, exoglucanase and β-glucosidase. Processivity is a common mode of action for many exoglucanases and plays a key role in the complete hydrolysis of crystalline cellulose. In contrast, classic endoglucanases randomly cleave β-1,4-glycosidic bonds in the interior of the cellulosic chains and have been considered to be non-processive. Studies have shown that in contrast to other cellulolytic systems that dependent on both endo and exoglucanases, a processive endoglucanase coupled with a β-glucosidase may be sufficient for the degradation of cellulose. Cellulase EG5C is a kind of proteins found from Bacillus subtilis 

BS-5 (1). It is hypothesized that

B. subtilis BS-5 might produce a processive endoglucanase, which might substitute for the apparent deficiency in exoglucanase activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 958
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 610
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 620