Difference between revisions of "Part:BBa K257006:Design"
(→‎Design Notes)
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# does not require any modification of the cleavage site during the secretion process, in contrast to the polipoprotein
 
# does not require any modification of the cleavage site during the secretion process, in contrast to the polipoprotein
  
−
C-term fusion using RFC23 (siver)
+
C-term fusion using RFC23 (silver)
  
 
===Source===
 
===Source===

Revision as of 13:24, 21 October 2009

ompA sequence signal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amplified from the secretion vector pIN-III-ompA-2 (Ghrayeb et al, 1984) keeping the RBS

  1. functions efficiently to secrete a large amount of the OmpA protein
  2. does not require any modification of the cleavage site during the secretion process, in contrast to the polipoprotein

C-term fusion using RFC23 (silver)

Source

pIN-III-ompA-2

References

Ghrayeb et al 1984 - "Secretion Cloning Vectors in Escherichia coli" The EMBO Journal 3(10):2437-2442