Difference between revisions of "Part:BBa K243006:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
 
Glycine and serine are good suitable for repetitive sequences. The properties zwitterionic and hydrophile prevent the aggregation of the linker.<br>The sequence has no cleavage site for proteases. <br>
 
Glycine and serine are good suitable for repetitive sequences. The properties zwitterionic and hydrophile prevent the aggregation of the linker.<br>The sequence has no cleavage site for proteases. <br>
−
Designed for the fusion of proteins or peptides via in frame cloning, according to RFC25.<br>
+
Designed for the fusion of proteins or peptides via in frame cloning, according to [https://parts.igem.org/Assembly_standard_25 RFC 25].<br>
 
[[Image:Freiburg09 Longlipart.png|750x550px]]<br>
 
[[Image:Freiburg09 Longlipart.png|750x550px]]<br>
 
[https://static.igem.org/mediawiki/parts/8/84/LongLinker.txt Commented GenBank file]
 
[https://static.igem.org/mediawiki/parts/8/84/LongLinker.txt Commented GenBank file]

Revision as of 13:15, 21 October 2009

Long Linker (Gly-Gly-Ser-Gly)x3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Glycine and serine are good suitable for repetitive sequences. The properties zwitterionic and hydrophile prevent the aggregation of the linker.
The sequence has no cleavage site for proteases.
Designed for the fusion of proteins or peptides via in frame cloning, according to RFC 25.
Freiburg09 Longlipart.png
Commented GenBank file

Source

Oligonucleotides designed by Team Freiburg Bioware09 and synthesized by sigma. Hybridized by PCR.

References