Difference between revisions of "Part:BBa K243004"

Revision as of 13:03, 21 October 2009

Short Linker (Gly-Gly-Ser-Gly)

This part is a linker, it can be used to connect two parts and add additional space between these parts. That can be necessary to avoid interactions between these parts. We used this part to connect our protein domains with our FluA and DigA domains, for our project the universal endonuclease.The sequence produced the aminoacids Gly-Gly-Ser-Gly.

Usage and Biology

Linker were used normally to connect two peptides, it is important for that connection,that the linker itself has no influence of the connected peptides. So the sequence of the linker is designed for amino acids which not interact with the environment. The amino acids glycine and serine are zwitterionic and hydrophile, these properties make them a good choice for the repetitive sequence of the linker.
The length of the linker is important to guarantee the independent function of two connected parts. When the linker is too short there might be a sterical interference between the parts and when it is too long, it can cause an instability of the construct.Also it is important that the linker has a certain flexibility.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K243004 short</partinfo>
-This part is a linker, it can be used to connect two parts and add additional space between these parts. That can be necessary to avoid interactions between these parts. We used this part to connect our protein domains with our FluA and DigA domains, for our project the universal endonuclease.The sequence produced the aminoacids Gly-Gly-Ser-Gly.
+This part is a linker, it can be used to connect two parts and add additional space between these parts. That can be necessary to avoid interactions between these parts. We used this part to connect our protein domains with our [https://parts.igem.org/Part:BBa_K157004 FluA] and [https://parts.igem.org/Part:BBa_K243003 DigA] domains, for our project the universal endonuclease.The sequence produced the aminoacids Gly-Gly-Ser-Gly.
 ===Usage and Biology===
-Linker are used to connect two parts. The created distance, between these two parts can guarantee independence
-function of the parts. The length of the linker is important to reduce the sterical interference between two parts.
+Linker were used normally to connect two peptides, it is important for that connection,that the linker itself has no influence of the connected peptides. So the sequence of the linker is designed for amino acids which not interact with the environment. The amino acids glycine and serine are zwitterionic and hydrophile, these properties make them a good choice for the repetitive sequence of the linker.<br>
+The length of the linker is important to guarantee the independent function of two connected parts. When the linker is too short there might be a sterical interference between the parts and when it is too long, it can cause an instability of the construct.Also it is important that the linker has a certain flexibility.
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