Difference between revisions of "Part:BBa K5143007"
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<img src="https://static.igem.wiki/teams/5143/bba-k5143007-cbd-cexv1-1.png" width="400" alt="CBD_CexV1"> | <img src="https://static.igem.wiki/teams/5143/bba-k5143007-cbd-cexv1-1.png" width="400" alt="CBD_CexV1"> | ||
<figcaption>Figure 1: ScCBD_CexV1 three-dimensional structure</figcaption> | <figcaption>Figure 1: ScCBD_CexV1 three-dimensional structure</figcaption> | ||
| + | </figure> | ||
| + | <figure> | ||
| + | <img src="https://static.igem.wiki/teams/5143/bba-k5143007-cbd-cexv1-2.png" width="200" alt="Functional cellulose"> | ||
| + | <figcaption>Figure 2: Functional cellulose</figcaption> | ||
<h1>Construction</h1> | <h1>Construction</h1> | ||
<p> | <p> | ||
Revision as of 09:28, 15 September 2024
Description
ScCBD_cex'V1 (Cellulose Binding Domain) is a peptide that has a huge affinity for bacterial cellulose. When fused with another protein, ScCBD_cex'V1 enables the fixation of the protein on the bacterial cellulose.
Construction
ECRIRE DES TRUCS ICI POUR LA CONSTRUCTION
References
Ong, E., Gilkes, N. R., Miller, R. C., Warren, R. A. & Kilburn, D. G. Te cellulose-binding domain (CBD(Cex)) of an exoglucanase from Cellulomonas fmi: production in Escherichia coli and characterization of the polypeptide. Biotechnol. Bioeng. 42, 401–409 (1993). Gilbert, C.; Tang, T.-C.; Ott, W.; Dorr, B. A.; Shaw, W. M.; Sun, G. L.; Lu, T. K.; Ellis, T. Living Materials with Programmable Functionalities Grown from Engineered Microbial Co-Cultures. Nat. Mater. 2021, 20 (5), 691–700. https://doi.org/10.1038/s41563-020-00857-5.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 256
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 226
Illegal AgeI site found at 265 - 1000COMPATIBLE WITH RFC[1000]