Difference between revisions of "Part:BBa K5492200:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
Codon optimisation for the following codons: AGA -> CGT, AGG -> CGT, ATA -> ATC, CCC -> CCG, CGG -> GCT, CTA -> CTG, GGA -> GGC
+
Codon optimised in summary of the following articles:
  
 +
Dong H, Nilsson L, Kurland CG. Co-variation of tRNA abundance and codon usage in Escherichia coli at different growth rates. J Mol Biol. 1996 Aug 2;260(5):649-63. doi: 10.1006/jmbi.1996.0428. PMID: 8709146.
  
 +
Lipinszki Z, Vernyik V, Farago N, Sari T, Puskas LG, Blattner FR, Posfai G, Gyorfy Z. Enhancing the Translational Capacity of E. coli by Resolving the Codon Bias. ACS Synth Biol. 2018 Nov 16;7(11):2656-2664. doi: 10.1021/acssynbio.8b00332. Epub 2018 Nov 2. PMID: 30351909.
  
 
===Source===
 
===Source===

Revision as of 08:38, 15 September 2024


Diamine oxidase - DAO


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 55
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 55
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1932
    Illegal BamHI site found at 2186
    Illegal XhoI site found at 802
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 55
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 55
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimised in summary of the following articles:

Dong H, Nilsson L, Kurland CG. Co-variation of tRNA abundance and codon usage in Escherichia coli at different growth rates. J Mol Biol. 1996 Aug 2;260(5):649-63. doi: 10.1006/jmbi.1996.0428. PMID: 8709146.

Lipinszki Z, Vernyik V, Farago N, Sari T, Puskas LG, Blattner FR, Posfai G, Gyorfy Z. Enhancing the Translational Capacity of E. coli by Resolving the Codon Bias. ACS Synth Biol. 2018 Nov 16;7(11):2656-2664. doi: 10.1021/acssynbio.8b00332. Epub 2018 Nov 2. PMID: 30351909.

Source

The source of the part is its amino acid sequence which was retrieved from Uniprot (https://www.uniprot.org/uniprotkb/Q498N2/entry#sequences), and the work of iGEM20_SEHS-China (Part: BBa_K3684002) which provided the dna sequence we improved on.

References