Difference between revisions of "Part:BBa K5036021"
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==Usage==
 
==Usage==
  
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This part induces target gene expression when functional dCas9/ VP64 reaches the promoter region by gRNA then it is recruited to promoter region causing more gene expression  
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This part is fused with GAL4, dcas9(c), VP64. it acts as a powerful transcription activator where it induce transcription and increased gene expression so after receptor activation, it will be directed with dCas9,VP64 and GAL4 to increases expression of YAP.
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 08:17, 14 September 2024


UAS Trans CMV enhancer

Part Description

It is CMV trans-enhancer protein is a molecule made by the cytomegalovirus (CMV) that works like a power switch for genes. It binds to specific DNA regions near genes, dramatically increasing their activity.

Usage

This part is fused with GAL4, dcas9(c), VP64. it acts as a powerful transcription activator where it induce transcription and increased gene expression so after receptor activation, it will be directed with dCas9,VP64 and GAL4 to increases expression of YAP. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]