Difference between revisions of "Part:BBa K5226022"
 
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<partinfo>BBa_K5226022 short</partinfo>
 
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===Sequence and Features===
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<partinfo>BBa_K5226022 SequenceAndFeatures</partinfo>
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===Introduction===
 
===Introduction===
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This part is a native ribosomal binding site of TD-sdaA, which has not yet been characterized. We assessed the ease of plasmid construction and ultimately chose to utilize this part in the composite parts(BBa_xx) to facilitate the expression of L-serine dehydratase.
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One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain <i>Halomonas</i> TD strains that can <b>metabolize formate</b>. We chose to <b>introduce the formate assimilation pathway</b> to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, <b>based on previous studies obtained from literature research</b>, we <b>selected the tetrahydrofolate (THF) cycle imported from <i>Methylobacterium extorquens</i> AM1 and strengthened the endogenous C2 and C3 modules of <i>Halomonas</i> TD</b>.
  
 
===Usage and Biology===
 
===Usage and Biology===
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This part is a native ribosomal binding site of TD-sdaA, which has not yet been characterized. We assessed the ease of plasmid construction and ultimately chose to utilize this part in the composite parts(BBa_xx) to facilitate the expression of L-serine dehydratase.
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This part is a <b>native ribosomal binding site of TD-sdaA</b>, which has not yet been characterized. We assessed the ease of plasmid construction and ultimately chose to utilize this part in the composite parts to <b>facilitate the expression of L-serine dehydratase</b>.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K5226022 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 02:27, 14 September 2024


RBS for TD-sdaA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Introduction

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1 and strengthened the endogenous C2 and C3 modules of Halomonas TD.

Usage and Biology

This part is a native ribosomal binding site of TD-sdaA, which has not yet been characterized. We assessed the ease of plasmid construction and ultimately chose to utilize this part in the composite parts to facilitate the expression of L-serine dehydratase.