Difference between revisions of "Part:BBa K5226009"

 
 
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<partinfo>BBa_K5226009 short</partinfo>
 
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===Sequence and Features===
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<partinfo>BBa_K5226009 SequenceAndFeatures</partinfo>
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One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. As a second approach, based on the homology between Vibrio natriegens and Halomonas TD, we chose to import the C1, C2, and C3 modules from Vibrio natriegens (parts:).
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===Introduction===
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One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain <i>Halomonas</i> TD strains that can <b>metabolize formate</b>. We chose to <b>introduce the formate assimilation pathway</b> to enable it to utilize formate, a difficult-to-recover product in CDE. As a second approach, <b>based on the homology between <i>Vibrio natriegens</i> and <i>Halomonas</i> TD</b>, we chose to <b>import the C1, C2, and C3 modules from <i>Vibrio natriegens</i></b>.
  
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===Usage and Biology===
 
===Usage and Biology===
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Vib-Ftl is <b>formate-tetrahydrofolate ligase</b>. The protein sequence is from <i>Vibrio natriegens</i> NBRC 15636 = ATCC 14048 = DSM 759. This protein <b>catalyzes the formation of 10-formyltetrahydrofolate from formate and  tetrahydrofolate during the tetrahydrofolate (THF) cycle(C1M)</b> .
  
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<span class='h3bb'>Sequence and Features</span>
 
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Latest revision as of 09:22, 13 September 2024

Vib-ftl

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1059
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1059
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1059
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1059
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1059
    Illegal NgoMIV site found at 325
  • 1000
    COMPATIBLE WITH RFC[1000]


Introduction

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. As a second approach, based on the homology between Vibrio natriegens and Halomonas TD, we chose to import the C1, C2, and C3 modules from Vibrio natriegens.

Usage and Biology

Vib-Ftl is formate-tetrahydrofolate ligase. The protein sequence is from Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759. This protein catalyzes the formation of 10-formyltetrahydrofolate from formate and tetrahydrofolate during the tetrahydrofolate (THF) cycle(C1M) .