Difference between revisions of "Part:BBa K5317006"
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<partinfo>BBa_K5317006 short</partinfo> | <partinfo>BBa_K5317006 short</partinfo> | ||
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| + | ===Usage and Biology=== | ||
The MRE-sites containing promoter enables the metal-dependent expression of a downstream positioned reporter gene via the metal ion-dependent transcription factor MTF-1 for cell-based metal detection. | The MRE-sites containing promoter enables the metal-dependent expression of a downstream positioned reporter gene via the metal ion-dependent transcription factor MTF-1 for cell-based metal detection. | ||
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To combine the observations made by Searle and colleagues and Wang and colleagues in 1985 and 2004, respectively, regarding the metal inducibility of a promoter with two MREa sites and a high affinity between MREd and MTF-1, we designed a synthetic promoter with two MREa and two MREd sites that alternate. The aim is possibly increasing the sensitivity and efficiency of the metal-dependent promoter. | To combine the observations made by Searle and colleagues and Wang and colleagues in 1985 and 2004, respectively, regarding the metal inducibility of a promoter with two MREa sites and a high affinity between MREd and MTF-1, we designed a synthetic promoter with two MREa and two MREd sites that alternate. The aim is possibly increasing the sensitivity and efficiency of the metal-dependent promoter. | ||
| + | =Cloning= | ||
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| − | + | ===Theoretical Part Design=== | |
| − | + | The MREdada promoter sequence was synthesized in order to insert it into the EGFP-C2 backbone (registry entry K3338020) for promoter efficiency testing. | |
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| + | ===Sequence and Features=== | ||
<partinfo>BBa_K5317006 SequenceAndFeatures</partinfo> | <partinfo>BBa_K5317006 SequenceAndFeatures</partinfo> | ||
Revision as of 09:20, 13 September 2024
MREdada promoter
Usage and Biology
The MRE-sites containing promoter enables the metal-dependent expression of a downstream positioned reporter gene via the metal ion-dependent transcription factor MTF-1 for cell-based metal detection.
To combine the observations made by Searle and colleagues and Wang and colleagues in 1985 and 2004, respectively, regarding the metal inducibility of a promoter with two MREa sites and a high affinity between MREd and MTF-1, we designed a synthetic promoter with two MREa and two MREd sites that alternate. The aim is possibly increasing the sensitivity and efficiency of the metal-dependent promoter.
Cloning
Theoretical Part Design
The MREdada promoter sequence was synthesized in order to insert it into the EGFP-C2 backbone (registry entry K3338020) for promoter efficiency testing.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]