Difference between revisions of "Part:BBa K5226008"
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K5226008 short</partinfo>
 
<partinfo>BBa_K5226008 short</partinfo>
−
 
+
===Sequence and Features===
 +
<partinfo>BBa_K5226008 SequenceAndFeatures</partinfo>
 +
<br>
 
===Introduction===
 
===Introduction===
−
One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1(parts:) and strengthened the endogenous C2 and C3 modules of Halomonas TD(parts:).
+
One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain <i>Halomonas</i> TD strains that can <b>metabolize formate</b>. We chose to <b>introduce the formate assimilation pathway</b> to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, <b>based on previous studies obtained from literature research</b>, we <b>selected the tetrahydrofolate (THF) cycle imported from <i>Methylobacterium extorquens</i> AM1 and strengthened the endogenous C2 and C3 modules of <i>Halomonas</i> TD</b>.
 +
 
  
 
===Usage and Biology===
 
===Usage and Biology===
−
TD-SdaA is L-serine dehydratase.The protein sequence is from Halomonas sp. TD01 strain.This protein catalyzes the conversion of L-serine into pyruvate during the glycine assimilation(C3M) . This part is used in BBa_xx and BBa_xx.
+
TD-SdaA is <b>L-serine dehydratase<ib>.The protein sequence is from <i>Halomonas sp.</b> TD01 strain.This protein <b>catalyzes the conversion of L-serine into pyruvate during the glycine assimilation(C3M)</b> .
−
 
+
−
<!-- -->
+
−
<span class='h3bb'>Sequence and Features</span>
+
−
<partinfo>BBa_K5226008 SequenceAndFeatures</partinfo>
+
−
 
+
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 09:18, 13 September 2024

TD-sdaA

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1119
    Illegal SpeI site found at 646
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 646
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1119
    Illegal SpeI site found at 646
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1119
    Illegal SpeI site found at 646
  • 1000
    COMPATIBLE WITH RFC[1000]


Introduction

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1 and strengthened the endogenous C2 and C3 modules of Halomonas TD.


Usage and Biology

TD-SdaA is L-serine dehydratase<ib>.The protein sequence is from Halomonas sp.</b> TD01 strain.This protein <b>catalyzes the conversion of L-serine into pyruvate during the glycine assimilation(C3M)</b> .