Difference between revisions of "Part:BBa K5226005"

 
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K5226005 short</partinfo>
 
<partinfo>BBa_K5226005 short</partinfo>
 +
===Sequence and Features===
 +
<partinfo>BBa_K5226005 SequenceAndFeatures</partinfo>
 +
<br>
 +
===Introduction===
 +
One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain <i>Halomonas</i> TD strains that can <b>metabolize formate</b>. We chose to <b>introduce the formate assimilation pathway</b> to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, <b>based on previous studies obtained from literature research</b>, we <b>selected the tetrahydrofolate (THF) cycle imported from <i>Methylobacterium extorquens</i> AM1 and strengthened the endogenous C2 and C3 modules of <i>Halomonas</i> TD</b>.
  
One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1(parts:) and strengthened the endogenous C2 and C3 modules of Halomonas TD(parts:).
 
  
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
TD-GcvH is <b>glycine cleavage system protein H</b>.The protein sequence is from <i>Halomonas sp.</i> TD01 strain.The H protein <b>shuttles the methylamine group of glycine from the P protein to the T protein during the glycine cleavage system(C2M)</b> .
  
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K5226005 SequenceAndFeatures</partinfo>
 
  
  

Latest revision as of 09:12, 13 September 2024

TD-gcvH

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 216
  • 1000
    COMPATIBLE WITH RFC[1000]


Introduction

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1 and strengthened the endogenous C2 and C3 modules of Halomonas TD.


Usage and Biology

TD-GcvH is glycine cleavage system protein H.The protein sequence is from Halomonas sp. TD01 strain.The H protein shuttles the methylamine group of glycine from the P protein to the T protein during the glycine cleavage system(C2M) .