Difference between revisions of "Part:BBa K5226004"
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K5226004 short</partinfo>
 
<partinfo>BBa_K5226004 short</partinfo>
−
 
+
===Sequence and Features===
 +
<partinfo>BBa_K5226004 SequenceAndFeatures</partinfo>
 +
<br>
 
===Introduction===
 
===Introduction===
−
One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1(parts:) and strengthened the endogenous C2 and C3 modules of Halomonas TD(parts:).
+
One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain <i>Halomonas</i> TD strains that can <b>metabolize formate</b>. We chose to <b>introduce the formate assimilation pathway</b> to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, <b>based on previous studies obtained from literature research</b>, we <b>selected the tetrahydrofolate (THF) cycle imported from <i>Methylobacterium extorquens</i> AM1 and strengthened the endogenous C2 and C3 modules of <i>Halomonas</i> TD</b>.
  
 
===Usage and Biology===
 
===Usage and Biology===
−
TD-GcvT is Aminomethyltransferase.The protein sequence is from Halomonas sp. TD01 strain.This protein catalyzes the conversion of S-aminomethyl-dihydrolipoyllysine into dihydrolipoyllysine during the glycine cleavage system(C2M) . This part is used in BBa_xx and BBa_xx.
+
TD-GcvT is <b>Aminomethyltransferase</b>.The protein sequence is from <i>Halomonas sp.</i> TD01 strain.This protein <b>catalyzes the conversion of S-aminomethyl-dihydrolipoyllysine into dihydrolipoyllysine during the glycine cleavage system(C2M)</b> .  
  
−
<!-- -->
 
−
<span class='h3bb'>Sequence and Features</span>
 
−
<partinfo>BBa_K5226004 SequenceAndFeatures</partinfo>
 
  
  

Latest revision as of 09:11, 13 September 2024

TD-gcvT

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 436
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 627
    Illegal PstI site found at 436
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 436
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 436
  • 1000
    COMPATIBLE WITH RFC[1000]


Introduction

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1 and strengthened the endogenous C2 and C3 modules of Halomonas TD.

Usage and Biology

TD-GcvT is Aminomethyltransferase.The protein sequence is from Halomonas sp. TD01 strain.This protein catalyzes the conversion of S-aminomethyl-dihydrolipoyllysine into dihydrolipoyllysine during the glycine cleavage system(C2M) .