Difference between revisions of "Part:BBa K5036019"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K5036019 short</partinfo> | <partinfo>BBa_K5036019 short</partinfo> | ||
+ | ==Part Description== | ||
it is a non structural large and multifunctional protein which is encoded by coronavirus. NSP3 plays a crucial role in viral replication through its different activities as protease . Studying NSP3 enables the researchers to gain data about the mechanism of SARS-Cov2 infection and disease progression . | it is a non structural large and multifunctional protein which is encoded by coronavirus. NSP3 plays a crucial role in viral replication through its different activities as protease . Studying NSP3 enables the researchers to gain data about the mechanism of SARS-Cov2 infection and disease progression . | ||
+ | ==Usage== | ||
+ | We inserted this part to be attached to the cap of linear RNA.After cutting the poly A tail, the aptamer will be expressed to be attached to MCP. Once the injury occurs ,MMP9 increases and it will mediate the circularization of RNA through its nanobody. | ||
+ | <html><div align="center"style="border:solid #17252A; width:100%;float:center;"><img style=" max-width:850px; | ||
+ | width:75%; | ||
+ | height:auto; | ||
+ | position: relative; | ||
+ | top: 50%; | ||
+ | left: 35%; | ||
+ | transform: translate( -50%); | ||
+ | padding-bottom:25px; | ||
+ | padding-top:25px; | ||
+ | "src="https://static.igem.wiki/teams/5036/parts/nsp3-attia.png | ||
+ | "> | ||
+ | <p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span | ||
+ | lang=EN style='font-size:11.0pt;line-height:115%'>this figure illustrates the structure of NSP3 in our switch | ||
+ | . </span></p></div></html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 15:50, 12 September 2024
MS2(x24)-HHR
Part Description
it is a non structural large and multifunctional protein which is encoded by coronavirus. NSP3 plays a crucial role in viral replication through its different activities as protease . Studying NSP3 enables the researchers to gain data about the mechanism of SARS-Cov2 infection and disease progression .
Usage
We inserted this part to be attached to the cap of linear RNA.After cutting the poly A tail, the aptamer will be expressed to be attached to MCP. Once the injury occurs ,MMP9 increases and it will mediate the circularization of RNA through its nanobody.
this figure illustrates the structure of NSP3 in our switch .
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 7
Illegal XhoI site found at 1 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 20
Illegal AgeI site found at 238
Illegal AgeI site found at 456 - 1000COMPATIBLE WITH RFC[1000]