Difference between revisions of "Part:BBa K283014"
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<partinfo>BBa_K283014 short</partinfo> | <partinfo>BBa_K283014 short</partinfo> | ||
| − | We intended to use this device to control the expression level of cheZ.BBa_K094141 was used to repress pLacI promoter. But we failed because of the high leaky expression. | + | We intended to use this device to control the expression level of cheZ.BBa_K094141 was used to repress pLacI promoter. But we failed because of the high leaky expression. This part does not pose any biological threat. |
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Revision as of 11:58, 21 October 2009
pLacIq-rbs-lacI-T-pLacI-rbs-cheZ_histag-double terminators
We intended to use this device to control the expression level of cheZ.BBa_K094141 was used to repress pLacI promoter. But we failed because of the high leaky expression. This part does not pose any biological threat.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2196
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1261
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]