Difference between revisions of "Part:BBa K5036005"

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==Usage==
 
==Usage==
 
This part is fused with GAL4, dcas9(c).  it acts as a powerful transcription activator where it induce transcription and increased gene expression so therefore it increases expression of YAP.
 
This part is fused with GAL4, dcas9(c).  it acts as a powerful transcription activator where it induce transcription and increased gene expression so therefore it increases expression of YAP.
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<html><div align="center"style="border:solid #17252A; width:100%;float:center;"><img style="                              max-width:850px;
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width:75%;
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height:auto;
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position: relative;
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top: 50%;
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left: 35%;
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transform: translate( -50%);
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padding-bottom:25px;
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padding-top:25px;
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"src="https://static.igem.wiki/teams/5036/parts/vp64-attia.png
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">
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<p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span
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lang=EN style='font-size:11.0pt;line-height:115%'>this figure illustrates the structure and function of VP64
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.  </span></p></div></html>
  
 
==literature characterization==
 
==literature characterization==

Revision as of 06:39, 12 September 2024


VP64

Part Description

It is a transcriptional activator composed of four tandem copies of VP16 (Herpes Simplex Viral Protein 16, amino acids 437-447*: DALDDFDLDML) connected with glycine-serine (GS) linkers. When fused to another protein domain that can bind near the promoter of a gene, VP64 acts as a strong transcriptional activator

Usage

This part is fused with GAL4, dcas9(c). it acts as a powerful transcription activator where it induce transcription and increased gene expression so therefore it increases expression of YAP.

this figure illustrates the structure and function of VP64 .

literature characterization

vp64 were transfected into HEK293T reporter cells and treated with abscisic acid or DMSO for 48 h.

Statistical significance was calculated with one-way ANOVA, using Dunnett’s multiple testing correction which showed that GFP expression was higher with VP64 than with any other promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]