Difference between revisions of "Part:BBa K5036003"
(→Usage) |
|||
| Line 5: | Line 5: | ||
This is the TEV Cleavage Site which is a particular amino acid sequence that the TEV protease detects and cleaves | This is the TEV Cleavage Site which is a particular amino acid sequence that the TEV protease detects and cleaves | ||
==Usage== | ==Usage== | ||
| − | This specific amino acid sequence is recognized by TEV, which can then cleave it to liberate dcas9 from our | + | This specific amino acid sequence is recognized by TEV, which can then cleave it to liberate dcas9 from our dCas9-synRTK receptor when VEGF binds to our receptor and activates it |
| + | |||
==literature characterization== | ==literature characterization== | ||
This research investigated how adding a TEV cleavage site impacted the production and solubility of nine specific mammalian proteins within bacterial cells. It's important to note that only these nine proteins were examined, and they are not representative of a broader selection. | This research investigated how adding a TEV cleavage site impacted the production and solubility of nine specific mammalian proteins within bacterial cells. It's important to note that only these nine proteins were examined, and they are not representative of a broader selection. | ||
Revision as of 17:51, 10 September 2024
TCS (Q, G)
Part Description
This is the TEV Cleavage Site which is a particular amino acid sequence that the TEV protease detects and cleaves
Usage
This specific amino acid sequence is recognized by TEV, which can then cleave it to liberate dcas9 from our dCas9-synRTK receptor when VEGF binds to our receptor and activates it
literature characterization
This research investigated how adding a TEV cleavage site impacted the production and solubility of nine specific mammalian proteins within bacterial cells. It's important to note that only these nine proteins were examined, and they are not representative of a broader selection. Four distinct tags were employed in the experiment: His-L, His-S, TEV1, and TEV2
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]