Difference between revisions of "Part:BBa K5226084"

 
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<h2>Sequence and Features</h2>
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<h2>Introduction</h2>
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In order to <b>produce multiple classes of high value-added bioproducts while absorbing assimilated CDE</b>, based on the current research of related literature, we iGEM24-SCUT-China-A chose to <b>introduce the synthesis pathway of PHA particle-associated protein.</b>
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<br>
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<h2>Usage and Biology</h2>
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The phaP gene produces a protein that <b>binds to the surface of hydrophobic polyhydroxyalkanoate (PHA) particles and plays an important role in regulating PHA synthesis and possibly protein folding.<b> To test the ability of <i>Halomonas</i> TD to produce phaP protein, we chose to use the inducible promoter Mmp1. In this experiment given, we added 50 mg/L of IPTG to the shake flask fermentation system for induced fermentations
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<h2>Experimental characterisation</h2>
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<h3>growth conditions</h3>
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<h3>shake flask studies</h3>
  
 
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Revision as of 12:48, 10 September 2024

Mmp1-phaP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Introduction


In order to produce multiple classes of high value-added bioproducts while absorbing assimilated CDE, based on the current research of related literature, we iGEM24-SCUT-China-A chose to introduce the synthesis pathway of PHA particle-associated protein.

Usage and Biology

The phaP gene produces a protein that binds to the surface of hydrophobic polyhydroxyalkanoate (PHA) particles and plays an important role in regulating PHA synthesis and possibly protein folding. To test the ability of Halomonas TD to produce phaP protein, we chose to use the inducible promoter Mmp1. In this experiment given, we added 50 mg/L of IPTG to the shake flask fermentation system for induced fermentations

Experimental characterisation

growth conditions

shake flask studies