Difference between revisions of "Part:BBa K5398006:Design"

 
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===Design Notes===
 
===Design Notes===
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none
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The ATG of TRn5 would be deleted in our actual work.
−
 
+
−
 
+
  
 
===Source===
 
===Source===
Line 16: Line 14:
  
 
===References===
 
===References===
 +
<p>[1] LIU L, WANG P, ZHAO D, et al. Engineering Circularized mRNAs for the Production of Spider Silk Proteins[J]. <i>Appl. Environ. Microbiol.</i>, 2022, 88(8): e00028-22.</p>
 +
<p>[2] PERRIMAN R, ARES M. Circular mRNA can direct translation of extremely long repeating-sequence proteins in vivo[J]. <i>RNA</i>, 1998, 4(9): 1047-1054.</p>
 +
<p>[3] LEE S O, XIE Q, FRIED S D. Optimized Loopable Translation as a Platform for the Synthesis of Repetitive Proteins[J]. <i>ACS Cent. Sci.</i>, 2021, 7(10): 1736-1750.</p>
 +
<p>[4] OBI P, CHEN Y G. The design and synthesis of circular RNAs[J].<i> Methods</i>, 2021, 196: 85-103.</p>
 +
<p>[5] GOMES R M O da S, SILVA K J G da, THEODORO R C. Group I introns: Structure, splicing and their applications in medical mycology[J].<i> Genet. Mol. Biol.</i>, 2024, 47: e20230228.</p>

Latest revision as of 09:32, 10 September 2024


pET29a(+)-cmRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 308
    Illegal XhoI site found at 861
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The ATG of TRn5 would be deleted in our actual work.

Source

none

References

[1] LIU L, WANG P, ZHAO D, et al. Engineering Circularized mRNAs for the Production of Spider Silk Proteins[J]. Appl. Environ. Microbiol., 2022, 88(8): e00028-22.

[2] PERRIMAN R, ARES M. Circular mRNA can direct translation of extremely long repeating-sequence proteins in vivo[J]. RNA, 1998, 4(9): 1047-1054.

[3] LEE S O, XIE Q, FRIED S D. Optimized Loopable Translation as a Platform for the Synthesis of Repetitive Proteins[J]. ACS Cent. Sci., 2021, 7(10): 1736-1750.

[4] OBI P, CHEN Y G. The design and synthesis of circular RNAs[J]. Methods, 2021, 196: 85-103.

[5] GOMES R M O da S, SILVA K J G da, THEODORO R C. Group I introns: Structure, splicing and their applications in medical mycology[J]. Genet. Mol. Biol., 2024, 47: e20230228.