Difference between revisions of "Part:BBa K5049001"
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| + | GCW61, one of the glycosylphosphatidylinositol-modified cell wall proteins (GPI-CWPs) in Pichia pastoris, plays a crucial role in maintaining normal morphology in yeast cells. This anchoring enable enzyme anchoring on the cell surface and performing functionality throughout feed processing, ensuring consistent performance in agricultural applications. | ||
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| + | The sequence of DNA elements for GCW61 anchor protein were from Pichia pastoris. To ensure flexibility in connecting to target fusion proteins, a GS linker ((GGSG)3) was added at the N-terminus of GCW61.The GS-GCW61 DNA fragment was synthesized by IDT and cloned into pSB1C3 as a basic part. | ||
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| − | <span class='h3bb'>Sequence and Features</span> | + | ===<span class='h3bb'>Sequence and Features</span>=== |
<partinfo>BBa_K5049001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K5049001 SequenceAndFeatures</partinfo> | ||
Latest revision as of 03:44, 10 September 2024
GS-GCW61 (GCW61 anchor protein with a GS linker)
GCW61, one of the glycosylphosphatidylinositol-modified cell wall proteins (GPI-CWPs) in Pichia pastoris, plays a crucial role in maintaining normal morphology in yeast cells. This anchoring enable enzyme anchoring on the cell surface and performing functionality throughout feed processing, ensuring consistent performance in agricultural applications.
The sequence of DNA elements for GCW61 anchor protein were from Pichia pastoris. To ensure flexibility in connecting to target fusion proteins, a GS linker ((GGSG)3) was added at the N-terminus of GCW61.The GS-GCW61 DNA fragment was synthesized by IDT and cloned into pSB1C3 as a basic part.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 187 - 1000COMPATIBLE WITH RFC[1000]